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mouse noggin cf chimera  (R&D Systems)


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    Structured Review

    R&D Systems mouse noggin cf chimera
    Mouse Noggin Cf Chimera, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+recombinant+noggin+fc+chimera/Recombinant+Mouse+Noggin+Fc+Chimera+Protein%2C+CF/pmc12510166-274-7-10
    Average 94 stars, based on 41 article reviews
    mouse noggin cf chimera - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Clonal Neural Stem Cells from Human Embryonic Stem Cell Colonies
    Article Snippet: Transgenic hESCs (provided by Jon Draper, McMaster University, Hamilton, Ontario, Canada) included H9-red fluorescent protein (RFP), CA1-RFP, and CA1-green fluorescent protein (GFP), which carry histone 2B/GFP, or histone 2B/RFP fusion proteins. hESCs were maintained on mitotically inactivated mouse embryonic fibroblast feeder layers in media containing 80% knock-out DMEM and 20% knock-out serum replacement (Invitrogen), 1% non-essential amino acids, 1 m m l -glutamine, 0.1 m m 2-mercaptoethanol, and 4 ng/ml basic fibroblast growth factor (FGF). .. Confluent colonies were disaggregated into ∼100 cell clumps using 0.1% type IV collagenase (Invitrogen) and passaged 1:3 or 1:4 every 4–7 d. For Noggin pretreatment, 100 ng/ml mouse recombinant Noggin/Fc chimera (R&D systems) was added to fresh media each day until colonies became confluent (4–7 d). .. Cell fluorescence was visualized using a motorized inverted microscope (IX81; Olympus) and a Fluoview Confocal Microscope (Olympus).

    Article Title: Clonal Neural Stem Cells from Human Embryonic Stem Cell Colonies
    Article Snippet: Transgenic hESCs (provided by Jon Draper, McMaster University, Hamilton, Ontario, Canada) included H9-red fluorescent protein (RFP), CA1-RFP, and CA1-green fluorescent protein (GFP), which carry histone 2B/GFP, or histone 2B/RFP fusion proteins. hESCs were maintained on mitotically inactivated mouse embryonic fibroblast feeder layers in media containing 80% knock-out DMEM and 20% knock-out serum replacement (Invitrogen), 1% non-essential amino acids, 1 mM L-glutamine, 0.1 mM 2-mercaptoethanol, and 4 ng/ml basic fibroblast growth factor (FGF). .. Confluent colonies were disaggregated into 100 cell clumps using 0.1% type IV collagenase (Invitrogen) and passaged 1:3 or 1:4 every 4 –7 d. For Noggin pretreatment, 100 ng/ml mouse recombinant Noggin/Fc chimera (R&D systems) was added to fresh media each day until colonies became confluent (4 –7 d). ..

    Article Title: Clonal Neural Stem Cells from Human Embryonic Stem Cell Colonies
    Article Snippet: Transgenic hESCs (provided by Jon Draper, McMaster University, Hamilton, Ontario, Canada) included H9-red fluorescent protein (RFP), CA1-RFP, and CA1-green fluorescent protein (GFP), which carry histone 2B/GFP, or histone 2B/RFP fusion proteins. hESCs were maintained on mitotically inactivated mouse embryonic fibroblast feeder layers in media containing 80% knock-out DMEM and 20% knock-out serum replacement (Invitrogen), 1% non-essential amino acids, 1 m m l -glutamine, 0.1 m m 2-mercaptoethanol, and 4 ng/ml basic fibroblast growth factor (FGF). .. Confluent colonies were disaggregated into ∼100 cell clumps using 0.1% type IV collagenase (Invitrogen) and passaged 1:3 or 1:4 every 4–7 d. For Noggin pretreatment, 100 ng/ml mouse recombinant Noggin/Fc chimera (R&D systems) was added to fresh media each day until colonies became confluent (4–7 d). hESCs were incubated at 37°C for 15–20 min in TrypLE Express for dissociation and then quenched with mouse embryonic fibroblast (MEF)-conditioned media before centrifugation. ..

    Incubation:

    Article Title: Clonal Neural Stem Cells from Human Embryonic Stem Cell Colonies
    Article Snippet: Transgenic hESCs (provided by Jon Draper, McMaster University, Hamilton, Ontario, Canada) included H9-red fluorescent protein (RFP), CA1-RFP, and CA1-green fluorescent protein (GFP), which carry histone 2B/GFP, or histone 2B/RFP fusion proteins. hESCs were maintained on mitotically inactivated mouse embryonic fibroblast feeder layers in media containing 80% knock-out DMEM and 20% knock-out serum replacement (Invitrogen), 1% non-essential amino acids, 1 m m l -glutamine, 0.1 m m 2-mercaptoethanol, and 4 ng/ml basic fibroblast growth factor (FGF). .. Confluent colonies were disaggregated into ∼100 cell clumps using 0.1% type IV collagenase (Invitrogen) and passaged 1:3 or 1:4 every 4–7 d. For Noggin pretreatment, 100 ng/ml mouse recombinant Noggin/Fc chimera (R&D systems) was added to fresh media each day until colonies became confluent (4–7 d). hESCs were incubated at 37°C for 15–20 min in TrypLE Express for dissociation and then quenched with mouse embryonic fibroblast (MEF)-conditioned media before centrifugation. ..

    Centrifugation:

    Article Title: Clonal Neural Stem Cells from Human Embryonic Stem Cell Colonies
    Article Snippet: Transgenic hESCs (provided by Jon Draper, McMaster University, Hamilton, Ontario, Canada) included H9-red fluorescent protein (RFP), CA1-RFP, and CA1-green fluorescent protein (GFP), which carry histone 2B/GFP, or histone 2B/RFP fusion proteins. hESCs were maintained on mitotically inactivated mouse embryonic fibroblast feeder layers in media containing 80% knock-out DMEM and 20% knock-out serum replacement (Invitrogen), 1% non-essential amino acids, 1 m m l -glutamine, 0.1 m m 2-mercaptoethanol, and 4 ng/ml basic fibroblast growth factor (FGF). .. Confluent colonies were disaggregated into ∼100 cell clumps using 0.1% type IV collagenase (Invitrogen) and passaged 1:3 or 1:4 every 4–7 d. For Noggin pretreatment, 100 ng/ml mouse recombinant Noggin/Fc chimera (R&D systems) was added to fresh media each day until colonies became confluent (4–7 d). hESCs were incubated at 37°C for 15–20 min in TrypLE Express for dissociation and then quenched with mouse embryonic fibroblast (MEF)-conditioned media before centrifugation. ..



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